We used stainless steel wire as electrodes, which seemed to work fine - at least for the weekend. We didn't see any noticeable corrosion after a few hours of running. You do have to make sure you really get stainless steel, not galvanized! The hardware store had 4-5 types of galvanized steel wire, but only one spool of stainless steel.
Graphite electrodes is a good idea. Of course, you still need to connect the graphite electrode to a wire somewhere, and you may need to protect that connection point if it's immersed in the liquid.
Patrik
On Thursday, November 8, 2012 3:31:54 AM UTC-8, Cathal wrote:
Nice work guys! I did love the knives-as-comb hack. :)--
What did you use for electrodes, though? Wouldn't do any harm once in a
while but if it were a regular party-piece, I'd invest in graphite
electrodes (artist's graphite pencils should do?). After
copper-containing electrodes used just once (at the DIYbio summit in
Manchester), I got to see just how much ionic metal gets dumped in the
buffer! Bright blue, frothy buffer!
On 06/11/12 00:57, Patrik D'haeseleer wrote:
> On Monday, November 5, 2012 4:49:40 PM UTC-8, Sebastian wrote:
>>
>> Would a clear soda like sprite have enough salt ions to work? Would be
>> cool if it worked. Im guessing the KCL used in your buffer was a multimolar
>> solution?
>>
>
> Sprite would probably work.Our solution was only very slightly salty to the
> taste, and I know most sodas include salts as taste enhancers. No idea what
> the carbonation will do though.
>
> Also, we didn't really care about buffering capacity of our "buffer", since
> we weren't woking with proteins or DNA where pH control really matters.
>
> Joseph wrote down all our recipes/protocols, so I'll wait for him to post
> those somewhere.
>
> Patrik
>
>
>
>
>
>> Either way, awesome hack. The stack of silverware as a comb was brilliant!
>> :)
>>
>> Sebastian S Cocioba
>> CEO & Founder
>> New York Botanics, LLC
>>
>> Sent via Mobile E-Mail
>>
>> On Nov 5, 2012, at 7:26 PM, "Patrik D'haeseleer" <pat...@gmail.com<javascript:>>
>> wrote:
>>
>> We wound up wasting a lot of time on the first day trying to get
>> electrophoresis to work on gelatin gels. We actually did get a tiny bit of
>> migration of the food dyes with a very solid gel (think "ballistics gel"
>> rather than jello!). But at lower gelatin concentrations that should have
>> given better migration into the gel, and would have been far more
>> palatable, the gels kept melting as soon as we applied current.
>>
>> Joseph Elsbernd (@CodonAUG) wound up bring much of his home lab to Science
>> Hack Day, so we did much of the initial experimentation on that, while we
>> worked to build a gel electrophoresis apparatus from scratch:
>>
>> <image.jpeg>
>>
>> And how's this for a gel comb hack. Yes, I know there are far easier ways
>> to make a gel comb, but this was more fun, and created lovely slated wells:
>>
>> <image.jpeg>
>>
>> Patrik
>>
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--
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