Agro injects into nucleus as far a I know. Normally plastid transformation is dun via gold gun. If you check the plos one article from the guy who made bioglow, it references his choice against agro for plastid transformation.
Sebastian S Cocioba
CEO & Founder
New York Botanics, LLC
Sent via Mobile E-Mail
Hi,
as I'll be getting Agrobacterium plasmids quite soon,
and there was that paper that said chloroplast transformation using agrobacterium is feasible,
do you think it would be worth a try?
Basically, I'd use an empty Ti plasmid, PCR a kanamycin resistance and lux operon within it.
I think, I wouldn't even need a promoter etc. because the chloroplasts can express operons. So in some of the transformed chloroplasts the gene cassete is inserted correctly between two genes of an operon.
Am I completely wrong?
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