Re: [DIYbio] Re: Excitation Spectra for Wildtype GFP?

Patents. >:(
Thanks. According to a ref on wikipedia, folding is impaired in the WT
protein at 37C, so hopefully growing at 30C will help prevent that.
Photostability is bad too, so will have to be sparing in my illumination
of the samples..

On 22/11/12 18:23, Josiah Zayner wrote:
> That should work.
> Any reason you used wtGFP and not one of the engineered versions?
>
> On Thursday, November 22, 2012 8:26:39 AM UTC-6, Cathal wrote:
>>
>> Hey all,
>> I'm going to be working with a construct containing wildtype GFP, with
>> the original peptide sequence from A.victoria, and I'm hoping someone
>> here would know whether it's feasible to excite with Blue light (which I
>> think you can do with normal GFP?) or if I'll have to use UV?
>>
>> Thanks
>> Cathal
>>
>

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