Ah I found it out!! At least I think so!!!
The 3' -> 5' has the correct sequence in the wrong orientation but it's correct if you read it in 5' -> 3'. This one is the template for the PCRs. Correct?
So I can just design the primers for this one and then it's like doing a PCR from 5' -> 3' strand
On Wed, Nov 21, 2012 at 3:40 PM, Andreas Sturm <masterstorm123@gmail.com> wrote:
Wait,
all genes go at the 5' -> 3' strand (clearly) and: always in 5' -> 3' direction?
I got a bit confused why the genome viewers then show them to be in reverse orientation "ATG-Gene-TAG" is then not "GAT-eneG-GTA" on the top strand??
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