Hi,
Yet a night-time only sollution seems more achievable
Why that? AFAIK we don't really know plant promoters that switch on when dark. So "always on" promoters (constitutively) would be the choice.
You know the difference between eukaryotic gene expression and prokaryotic one? Prokaryotes (Bacteria) can read entire operons (Promoter-GeneA-GeneB-GeneC-Terminator), while eukaryotes always need one promoter before each gene.
The only surely known light pathway is the lux operon from marine bacterie. It has also already been inserted into chloroplasts (they are ~bacterial endosymbionts in plant cells, so they can express operons)
You could get pVIB plasmid and insert it into E.Coli to get quick, yet very impressing, results.
Hope that helped a bit.
Also, there will be a geneome compiler kickstarter campaign which will synthesize many designs of glowing genes for plants then and try out which one works best.
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