Heres how to get the dna:
http://learn.genetics.utah.edu/content/labs/extraction/howto/Here is the general steps you take during the lab you are about to run
O*your dna fragment that you wanna put in the plant will be the dinoflagellates
O*your host will be the plant cell
-- The basic steps involved in producing recombinant DNA are outlined in Figure 1.
- Plan the cloning strategy. This includes selecting a method for obtaining the DNA fragment to be cloned, selecting a vector into which the DNA fragment will be incorporated, and selecting the bacterial host that will be used to produce the recombinant DNA.
- Isolate the vector and the DNA fragment to be cloned. For the vector this may involve growing an appropriate bacterial strain carrying the vector, and isolating the vector DNA. The method used to obtain the DNA for cloning depends on where it currently resides (in a plasmid, in genomic DNA, in mRNA etc.).
- Prepare the vector and the target DNA for ligation. The DNA that will be cloned needs to be an appropriate size for ligation (size range depends on the vector being used) and the vector needs to be opened up (made linear) so that the insert can be ligated to it.
- Ligate the digested vector with the DNA fragment(s) of interest.
- Screen the new plasmids to find the desired recombinant plasmid(s). This involves transforming host bacteria with the ligation mixture and using selection methods to isolate single bacteria carrying the newly created plasmids. Plasmids are then re-isolated and checked for correct structure.
 |
| Figure 1. Cloning Steps |
O*your host will be the plant cell
This is a very specific lab undertaking what you hope to do except with e.coli:
http://www.scribd.com/doc/14998428/Lab-6-Molecular-Biology
just read it, it's technical but if you fight thru it you may figure out how it applies to what you wanna do.
Heres a very specific article about scientist who have successfully done what you hope to do:
http://www.livescience.com/10307-transplanted-genes-plants-light.html
Make the new dna, put it in the plant, watch it glow.
of course it`s not gonna be that simple because you live nowhere near a hackerlab
but atleast you kan say you know how
On Thursday, December 27, 2012 9:10:07 PM UTC-5, T.J. wrote:
Mega,
Did you ever make a glowing bonsai tree? I would love to see pictures if you got them!
On Monday, December 24, 2012 12:59:01 AM UTC-5, TJ Reece wrote:Before I even try to make an attempt to have the slightest clue that I know what i'm talking about, or that I belong in the forum, I don't. While I've always been fascinated by science, my education in the field of bio is limited to a level 100 Biology course. However, since the movie "Avatar" came out I've had an interest in flourescent plants. Every now and then I'll refer back to google revising my search to look for bioluminous plants or any modifications that are currently on the market to apply your own. While Ideally I would like my garden plants to emit light constantly, or at a pulse like a firefly; Yet a night-time only sollution seems more achievable. I would love to see plants or grasses in my garden illuminate at night. The simplest sollution would be to paint the plants with UV ink, but I don't see this option to be practical.When I look into the field of genetic engineering WAY too much graduate level information comes up that I can't decipher. I have no problem reading a book or two to understand the concepts involved in a project like this, but I prefer more trial and error methods.I did find something that looks like it might be worth a try, but I would like to get a second opinion first. I saw this link human-plant hybrid of a youtube video that uses human hair and fern seeds to make the fern react to touch. It just seems that this video may be fake.After looking through various organisms that have bioluminous properties, I was wondering about dinoflagellates http://en.wikipedia.org/wiki/Dinoflagellate and whether it's possible using this method above to incorporate luminous properties into something like a fern seed. Seems a little way out of my education level to contemplate.If ANYONE on this board could give me any useful advise it would be much oblidged. Im unsure on what terms to search for, what books would be helpful, what equipment I would need. I could spend up to 1k, or maybe 2k on equipment AT MOST, but other than that I can't afford to finance the project. I've been looking at DNA synthesizers, Gene Guns, and other really expensive equipment that seems a little more over the top than what I may need, but still pretty cool stuff. Maybe I could start with plants and work my way up to mutating my own team of X-Men!!!MUAHAHAH (sorry, lol)
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