Thank you Mega and Dakota for your input,
The truth is, I only have a vague understanding of the theory behind what you're implying. And none whatsoever on the actual application process. I'm afraid I didn't emphasize enough on my rudimentary knowledge of biology in general. I understood prokaryotes to be single celled with no nucleus and eukaryotes to be the opposite(according to wikipedia this isn't always true). However I was able to google everything you wrote to get a common knowledge on the subject and am very thankul for your input. The question is now whether I would be able to pick up a few books and acquire the skill(s) related to do this, or if it's an accumulation of knowledge developed over years of practice?
Btw, I live in central Pennsylvania, Harrisburg area.
On Tuesday, December 25, 2012 4:49:38 AM UTC-5, Mega wrote:
Might I ask, where are you located?--
Another addition:
For all light emitting reactions you basically need luciferin and luciferase which react to produce light.
You saw the thread about the pre-coelenterazine pepdide? This is a luciferin, and Renilla luciferase (which is broadly available) is the luciferase. So you could build a full light-generating pathway using just these two genes (adding two strong promoters). For comparison, the vbacterial lux operon needs LuxA+B (Luciferase) and Lux C+D+E+G, so in total 6 genes.
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