I put that sequence into Genome compiler, Gentle and online compilers, and all showed the same (so no copy paste problem ;) ):
In the given sequence, there are Stop Codons, and once there is even TAA-ATG, so the ribosome will fall off in eukaryotes, while bacteria may be able to read this next open frame peptide too. (Is there no space needed inbetween?)
It's quite strange because it's not monomeric. Quite difficult to get the sequence I need, but in theory, I'd only need Renilla Luciferase and this Luciferin-making-peptide, put it into a plant nucleus.
On Tuesday, March 15, 2011 10:53:22 PM UTC+1, Cathal wrote:
--Brilliant though! That's something to work with at least. There are many things that could have been affecting things to slow down light emission. Codon optimization, protein separation, bottlenecking at any stage.. perhaps by codon optimizing and directly chaining proteins we could get it working? I saw linkage at work on a B12-13 pathway once, it was well over tenfold improvement by my recollection.
Time to read that paper!
On 15 Mar 2011 21:45, "Cory Tobin" <cory....@gmail.com> wrote:> As to bioluminescent plants, from my understanding they have never yet been
Sorry to interrupt the conversation on frankenstein experiments. One
> made to glow indepen...
small correction about the plants. There was one case where someone
built the entire luciferin synthesis pathway in plants.
http://dx.plos.org/10.1371/journal.pone.0015461 (PLoS One, open
access) Unfortunately the amount of luciferin it produced was so low
they could only see the autoluminescence with a five minute exposure
in the dark. So it wasn't visually stunning :[
-cory
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