phillyj and Dakota,
Thanks for your timely and informative replies.
The EZNA kit employs proteinase K which, as I understand it, acts in both lysis and protection from DNA degradation. Will you comment on that phillyj?
Another consideration to the EZNA kit is chloroform. That reagent is added to "remove much of the polysaccharides and proteins and improve spin-column performance." Regarding chloroform what I know is:
PVA gloves should be used in handling
As a gas it's exposure limit is 50ppm per 8 hour day.
It may decompose to phosgene in the presence of oxygen.
Stabilized by alcohol it has a shelf life of no more that 1 year.
Disposal is regulated
Anyone care to share their experience with chloroform?
My plan is to aliquot 500uL containers as that is the volume prescribed by the EZNA protocol. I'll do that under a fume hood in the lab. Having minimal aliquots should reduce the health and environmental risk given our amateur environment. Comments?
The Sticky Ends
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