I am fascinated by Cathal's approach to let the transformed organism produce its own "selection agent" while carrying the resistance gene.
That would surely work for chloroplasts too. The antibiotic would have to be exported out of the chloroplast, of course, so that it is not degraded wiithin the transformed plastid.
Plastid transformation is said to be quite difficult, because you have to get homoplastid-ic cells, otherwise the non-transformed chloroplasts may grow quicker than the transformesd ones and in the end there will be no transformed chloroplasts in the cell any more. With this approach however, they select theirselves!
Does anybody know by chance if there are gene clusters known that produce an antibiotic from stuff that is already present in each bacterium/plastid?
(e.g. Kanamycin, Streptomycin, .... ). I had a small google research on that, without usable results.
Unfortunately, there is no paper on the internet (I found so far) assessing the use of peptide antibiotics (like Nisin) against chloroplasts. Have to dig into the mechanism of destruction that is done by nisin, etc.
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