It's not just for long DNA strands. My background is structural biology (protein crystallography) - I can't count the time I spent constructing plasmids to try different combinations of expression tags/expression systems/stability elements/etc/etc. I wish I could spend my time designing and running experiments - but most of my time was wasted in PCR-cloning.
When I came to Stanford at 2007 the price of DNA was ~$2bp. when I left it was ~$0.39 so my lab moved to just ordering the genes already in the expression plasmids. If DNA was cheaper we would order much more (you never know what would crystalize - need to do tons of experiments) and would also order bigger things (a whole complexes, metabolic pathways, combinatorial libraries, etc). Cheaper DNA is a must in my opinion (and @#%@# those of little imagination that say they doesn't know what they will do with cheap DNA).
One reason we started Genome Compiler was for seeing that future coming - why else build a software from the ground up that can handle any size sequence?
On Sunday, December 9, 2012 7:33:33 AM UTC+2, Nathan McCorkle wrote:
Just wondering what researchers are limited today other than us DIYers who want to screw around with different DNA combinations. Who else uses long DNA strands?--
-Nathan
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