I know this may be overkill but would a PID controlled peltier work to control the heat generated from high current runs? Speed up gels and whatnot? Would we start delving into the land of agarose damage if the current is too high despite the relatively cool buffer? Lets say on top of the silly peltier we have a means of circulating the buffer also, could one make high speed gels like that? Just a thought.
Sebastian S Cocioba
CEO & Founder
New York Botanics, LLC
Sent via Mobile E-Mail
From what I've read constant current means constant migration speed, but as electrolysis occurs the resistance goes up and more heat is generated. Constant voltage means as resistance goes up due to electrolysis, current goes down and so does migration speed, but heat stays low.
On Dec 9, 2012 11:51 AM, "Jeswin" <phillyj101@gmail.com> wrote:--On Sat, Dec 8, 2012 at 9:47 PM, Nathan McCorkle <nmz787@gmail.com> wrote:
> On Sat, Dec 8, 2012 at 5:27 PM, Jeswin <phillyj101@gmail.com> wrote:
>>
>> I run at fixed current, 75V for 20-25 minutes (fresh, and larger gels)
>> and 50V for 35-45 minutes(older gels that were stored in 4C before
>> using).
>
>
> you mention fixed current, but then quote voltage specs. That would mean you
> control for gel resistance everytime you make a gel.
>
Ack. I meant voltage fixed at 75 or 50 and let the current vary. My
gelbox is a simple affair. It's lets you choose 6 or 7 different
voltages to run at and lets you program the timer.
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