Re: [DIYbio] Hacking Stink Bugs

Nice setup but do you have a UV lightbox to view the stained
electrophoresis gel?

Anyway, you can start with the primer design using BLAST to find the
appropriate sites.

As to isolation, is there anything different in insects that could
mess with purification of DNA?

And talking with suppliers, maybe you can say you are home-schooling
and working on this project. Maybe they can help you out if its some
kind of educational related.

On Thu, Dec 27, 2012 at 12:49 PM, Sticky Ends <julesorgeoff@gmail.com> wrote:
> Greetings,
>
>
>
> My 12 year-old daughter wants to be a molecular biologist. Sounds like
> quality time to me. I'm all in.
>
>
>
> The project is RFLP of stink bug genomic DNA (Halyomorpha halys). We not
> looking towards cutting edge research. We'll be stoked if we can isolate,
> restrict, electrophorese and visualize.
>
>
>
> I've collected some nice equipment including an electrophoresis system,
> centrifuge, pipettor, UV spectrophotometer and precision balance. All that
> wasn't cheap but I really want this project to work because success will be
> inspirational to the lass.
>
>
>
> Some questions to the community if I may?
>
>
>
> I plan to use spin-column kits for DNA isolation. In particular I like the
> EZNA kit from Omega Bio-tek. Any comment on this choice?
>
>
>
> Reagents are proving difficult to acquire. I understand why suppliers
> frustrate the process. But surely there is an avenue to quality reagents
> that is controlled yet accessible. Any advice on how to inform suppliers?
>
>
>
> Finally, as we are beginners, any other advice on getting started will be
> appreciated.
>
>
>
> Best regards,
>
>
>
> The Sticky Ends
>
> Maryland, USA
>
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