Re: [DIYbio] modified GFP -> pr-coelenterazine peptide

GFP really is just green, and fluorescent. However, in A.victoria, it's
complexed to a protein called Aquorin, which is a luciferase, and
produces blue light on digesting its luciferin. This blue light is
partially converted by GFP into green light, producing the turquoise
colour of the jellyfish's glow.

So, GFP on its own neither produces nor consumes a luciferin, but
Aquorin consumes a type of luciferin. However, Aquorin alone does not
produce or regenerate that luciferin, there's a separate pathway for that.

If memory serves, the luciferin for Aquorin is calcium-dependent, so it
is sensitive to membrane ion channels, etc.: I think I remember seeing
Aquorin used as an ionic indicator of some sort.

On 20/12/12 09:26, Andreas Sturm wrote:
> What I really wonder:
>
>
> Those jellyfish must produce coelenterazine, obviously, because they're
> glowing,
>
> So why the wild-type GFP doesn't produce the luciferin?
>
>
> I mean, there is just *one* amino acid (65 Ser -> Tyr) wrong in wild-type
> gfp, and I consider that highly unlikely that this gene is so very close to
> producing luciferin, but doesn't.
>
>
> Or, does the wild-type GFP produce coelenterazine in small amounts in
> vivio? Wouldn't this have been measured as GFP is used so frequently?
> Maybe irt's because one usually uses the enhanced GFP, and it does no
> longer make luciferin?
>

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