What I really wonder:
Those jellyfish must produce coelenterazine, obviously, because they're glowing,
So why the wild-type GFP doesn't produce the luciferin?
I mean, there is just one amino acid (65 Ser -> Tyr) wrong in wild-type gfp, and I consider that highly unlikely that this gene is so very close to producing luciferin, but doesn't.
Or, does the wild-type GFP produce coelenterazine in small amounts in vivio? Wouldn't this have been measured as GFP is used so frequently?
Maybe irt's because one usually uses the enhanced GFP, and it does no longer make luciferin?
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