If you look at the gyazo link I posted in my first response, that PCR was a result of genomic DNA taken from a mushroom. The initial sample size was maybe 1/2 the size of a pea, and I ground it for ~30 seconds with the end of a spatula in a 1.5 mL centriuge tube. There was still tons of debri left over during parts of the extraction (pipette as little debris as possible), but it was taken out after the first spin down. Ended up with a lot of decent genomic DNA from a tiny sample. Liquid nitrogen was going to be used, but the NMR tank was empty so...didn't get to use it.
I don't think liquid N2 is necessary, or for that matter even dry ice and acetone.
I see those dead pine beetle/stink bugs all the time at my dad's house up in maine. They do smell...strange! It smells like a mixture of ammonia and orange peels / citrus. Very cool, but it'd be awesome to GCMS it and see what it's made of!
For insects they say even a ground up leg has enough DNA.. Though I just remembered you aren't doing PCR....so perhaps cryogenic freezing prior to the extraction will increase yields. It may still be avoided though, give gDNA extraction without freezing the cells prior and see how much you get. I imagine it will be enough to do the experiments you want to. Out of a 200uL final elution volume of gDNA, only 2uL was used in the PCR rxn, so there's a lot left over. I don't know what kind of amounts of DNA a RFLP uses though, had to look up that acronym.
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