Re: [DIYbio] Re: Hacking Stink Bugs

On Thu, Dec 27, 2012 at 9:46 PM, Sticky Ends <julesorgeoff@gmail.com> wrote:
>
> Preparations of insect tissue are ideally made by cryogenically freezing and
> then grinding samples. I can achieve that but prefer the lesser
> alternatives. Any success stories using other mechanical methods such as
> straight mortar / pestle, coffee grinders or Dounce homogenizers?
>
I was thinking about this. N2 is usually a very fast way to grind them
down to a powder that you can extract DNA from. I think getting the
dead dry bugs like Dakota said will be just as good. No N2 needed,
just grind them down. I extracted DNA from 20mg of N2 crushed flax
plants. I did find that getting the right primers is difficult since
many regions are conserved. Finding that region that is unique to that
organism may or may not be trivial.

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