You are welcome, no problem, glad to help. I hope it didn't come off like I was trying to use MSDS fear propaganda to push you away from the spin columns. The concentrations and amounts used in both kits is by no means deadly, and the worst either of them might give you if a large amount was spilled on your skin is probably an annoying rash until washed with soap and water thoroughly.
For all I know, the Omega kit is more safe than the Wizard Promega kit. At first I was worried the wizard kit might not give enough DNA, but after checking their protocol handbook.
You can see that insect cells give 16ug. Compare that to a silica spin column which generally binds ~20ug DNA, and they are pretty similar.
I've only used spin kits before, and I'm sure what Wizard has made is a DNA extraction kit that mimics chloroform/isoamyl but without using those things, and without using silica columns. Spin columns might provide cleaner DNA to be honest, but I've never used the Wizard kit, so I can't compare.
As I see it, disregarding the chemical makeup of the solutions since handling and disposal will be treated equally
Omega Spin Kit
Pros:
5 isolation reactions for $16
silica binding column, greater DNA recovery and possibly "cleaner" than Wizard
Cons:
Require 100% non denatured ethanol to bring solutions to volume
Wizard non-spin kit
Pros:
Doesn't use silica columns (if you can call that a pro? I guess it'd make it cheaper)
Cons:
Needs 70% non-denatured ethanol
Needs 100% isopropanol
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So putting the MSDS stuff aside, because I don't want that to be the main factor, I honestly would go with the 5 reactions from Omega to start. It's $16 vs $150. I doubt you'll be needing a full Wizard kit with 100 isolations, so then having all that stuff lying around might even offset any danger one might feel reading the Omega spin kits MSDS.
You could use up the 5 spin kits from Omega and get your results, and not have leftover reagents.
Also, I just found a place where it seems you can get non-denatured ethanol for relativley cheap.
I think 25mL or 50 mL would be fine, because the Omega kit I think calls for 6 mL per 2 solvents or something like that, check out the protocol handbook on their website.
You can also get 190 proof alcohol at a liquor store called Everclear, though I don't know if it has other things in it that would screw up DNA recovery.
Spin kits generally call for 96%-100% ethanol to be used (non-denatured is key) and I can't imagine 95% would destroy your DNA recovery.
Also!!! I just remembered, the Wizard kit has you precipitate the final DNA in 100% isopropanol, then dry out the DNA to dryness, then re-suspend in a DNA re-hydration solution.
That solution has EDTA which could chelate to Mg2++ cofactor ions during your restriction enzyme digest and mess with results.
The pro about the spin kit is that you can elute into just plane water, which won't mess up your restriction digest.
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So to re-iterate, the 5 isolations for $16 + $30 for 190 proof ethanol would be a better buy then a $150 kit which probably won't ever be fully used, + even more money for 100% isopropanol.
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