Hi everybody.
I'd just like to know if you could possibly do a PCR from two sources at a time using three primers?
So e.g.:
Usually if you want to have a GFP-KanR construct, you would use four primers, with the primers between the two genes of interest having the same restriction site. Those would then be ligated later.
What if you took just one primer which contains the end of GFP + a short linker + beginning of KanR and of course one GPF starting primer and one KanR end primer? Would that still work?
Additionally, the sources were two different plasmids. Could that possibly work, to get one construct in one step (without the need of restriction digestion and re-ligating)
May it be a problem that the ends of primers can have a false sequence?
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To post to this group, send email to diybio@googlegroups.com.
To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio?hl=en.
To view this discussion on the web visit https://groups.google.com/d/msg/diybio/-/PiPKblieOsIJ.
For more options, visit https://groups.google.com/groups/opt_out.
0 comments:
Post a Comment