Plants generate a lot of oils (turpenes, isoprenoids, long-chain alcohols, etc). A lot of these are isolated by extraction with hydrophobic solvents with partition of non-oily components into an aqueous phase.
On Saturday, January 5, 2013 5:52:27 PM UTC-5, Josiah Zayner wrote:
Unless you need to run HPLC or TLC, which you most likely don't; I don't see the need for hydrophobic solvents. Furthermore, most people use Methanol, 2-Propanol or Acetonitrile all of which can be found on ebay.--
I have never heard of anyone boiling things off when doing a protein extraction.
Tissue homogenization can be done easily. Freeze tissue, add SDS or some detergent and grind, repeat.
Then one centrifuges and takes the soluble fraction the proteins can be separated using ammonium-chloride fractionation or something like ion-exchange chromatography that only requires salt and a syringe and a column.
On Saturday, January 5, 2013 4:16:41 PM UTC-6, Ulysses1994XF04 wrote:I've been wanting to build a small, home biochemistry lab for a while but the biggest thing holding me back from pouring the time and money into it is that I just can't find any place that will sell the reagents I want to use to individuals (most companies that make laboratory-grade reagents will only sell to certified labs at universities and companies and such).
I really want to do extractions of cellular products (enzymes, pigments, etc) of plants (grass clippings, dead flowers from my garden, etc) and fungi and bacteria I can just grow inside. For some of these, I need a good hydrophobic extraction solvent that's 1) as pure as possible and 2) has a low boiling point so I can easily evaporate/boil off (ideally, I would like to recapture it by distillation so I don't have to constantly buy more of it).
I can't find a single manufacturer that will sell chloroform, dichloromethane or small hydrocarbons like pentane and cyclopentane to individuals. What other hydrophobic solvents can I use?
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