Re: [DIYbio] 0.2 um filters for sterile filtering/possibly viscous solutions


> How do you know the plastic parts would not warp
> and cause leaks?

Only the vague recollection that the seller said they were sterilisable.

> Also, this is just the filter cartridge, you'd need to sterilize
> the whole assembly.

Yea, I was planning to just screw the thing into the bottom of a large container with an O-ring sealing with the bottom of the vessel, but I discovered to my dismay that the thread pitch on the fitting of the filter cartridge is really annoyingly non-standard, and it's hard to find a matching tap. So, as I had no pressing need to continue, I shelved it until TEOTWAWKI ( https://en.wikipedia.org/wiki/TEOTWAWKI ). :P

On 5 January 2013 19:59, Eugen Leitl <eugen@leitl.org> wrote:
On Sat, Jan 05, 2013 at 06:00:39PM +0000, Cathal Garvey wrote:
> I bought some ceramic "candle" filters for water sterilisation which would
> probably be good for sterilising large volumes of non-viscous liquid. You
> can autoclave them, AFAIK.

You're probably looking at http://www.amazon.de/Keramikfilter-Wasserfilter-NEU-Filter-Feinfilter/dp/B002RLKE7O/
or http://www.amazon.de/Keramikfilter-Kartusche-Wasserfilter-Partikelfilter-Filterkerze/dp/B00AAV53O2/

How do you know the plastic parts would not warp
and cause leaks?

Also, this is just the filter cartridge, you'd need to sterilize
the whole assembly.

> However, I've never had call to sterilise volumes large enough to test
> them; they are large enough that filter losses would be significant.

At volumes of 30+ liters, that's the least of my worry.

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