Re: [DIYbio] Re: Paper electrophoresis... super available molecule separation?

On 01/30/2013 05:49 PM, Nathan McCorkle wrote:
> But DNA migration depends on the e-field not on the power through it

Sure, that's true. And I was talking about the e-field also.
Ohms per square are the Ohms that resist the e-field. Look it up.

The aspect ratio of an electrophoresis path determines how conductive it is
if all other variables are the same, (salts, buffers, conductive stuff in solution,
gooeyness, amount of duct tape used, viscosity, Coriolis effect, what have you)

In short, neglect the rest, take the ohms per square analysis to get to the
heart of designing any new shape relative to a usual gel box.

How you reduce it to a 2D problem from a 3D world is to find the conductivity of a cube,
then map that onto your path layout in 2D and consider it a uniform layer that can be treated
as X,Y movement only, neglecting Z axis, and you're to a 2D representation that can
simplify and give you design insights.

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