If you can get your hands on some, try the filter paper used for Northern/Southern/Western blotting. Very fine grain, very uniform, and no contaminants.
On Wednesday, January 30, 2013 7:36:22 AM UTC-8, John Griessen wrote:
On 01/30/2013 07:48 AM, Dakota Hamill wrote:--
> Trying to think of other ways to seal off a narrow channel, to control the initial dispersion as you said, but keep open a running
> lane for the DNA or dye to migrate.
It seems to me that ordinary filter papers wick rapidly, making wide blurs out of any sample put on them.
If you coated filter paper with agarose then dried it be convenient paper again, it might
perform more like agarose -- low dispersion. If drying somehow ruins it,then store
it wet refrigerated/frozen and you'd get the agarose narrow dispersion
benefits with a small amount and the convenience of paper.
It might pay to try different papers. Clay coated papers for photo printing might be good.
Reading about TLC made me try searching for silica gel paper and it exists:
http://www.fishersci.com/ecomm/servlet/fsproductdetail_ 10652_785049__-1_0
and is thick, which is good for electrophoresis.
The electrophoresis voltage will have to be higher than with gel box agarose
since it is such a "paper thin" path, therefore less conductive for the same conditions.
Here is something from TLC:
http://85.238.144.18/lifescience/literature/061009_ Making_TLC_Plates_from_Bulk_ TLC_Silica_Gels.pdf
"The fine silica will not stay on the plate after coating if some type of binder is not
included. . . . The classical binder used is gypsum . . . In most silica gels it is put in
at a level of from 10-15% to give good binding to the glass plates . . . For a
stronger layer, polyvinyl alcohol or polyvinyl pyrollidone can be added to a TLC grade silica gel
(without other binders) in a level of 1-2% by weight as a polymeric binder . . . Heat drying after
air-drying makes cross-linking of this type of polymer binders.
What if you take fine filter paper strips, make a slurry as for TLC, pull paper through slurry
of silica gel and gypsum plaster, then a double squeegie, (like used to dry photo film),
to get a uniform amount on the paper?
Just some ideas. I can't stop to try these out myself just now.
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