Take a look at the video I linked to.
It shows how to extract limonene from orange peels using dry ice in a capped plastic bottle.
I've worked quite a bit with dichloromethane. It is not a friendly solvent. You'll want a fume hood or you'll want to perform the extraction outdoors. You don't want to breathe the vapors. Butane, however, is friendlier. It is non-toxic, and you breathe it right back out if you do inhale it. Smokers inhale it when they light up. Of course they also inhale much more toxic things that way, so that isn't much of an endorsement.
Dichloromethane leaves marks on anything is dissolves, and it dissolves a surprisingly large number of things in the typical house or basement lab. It can permanently fog your safety glasses, for example, depending on what they are made of.
And here is an interesting application of supercritical butane extraction that looks similar to what you want to do.
On Sat, Jan 5, 2013 at 6:07 PM, Ulysses1994XF04 <bensics@comcast.net> wrote:
Nathan McCorkle : "Maybe Simon or someone could weigh in on if this is easily DIYable, but supercritical CO2 is a great Non-Polar (NP) solvent."
Perhaps, but at room temp, CO2 is liquid only under extremely high pressure (a few dozen atmospheres). You'd probably need an heavy-duty air tight pressurized chamber, but then how would you manually manipulate the stuff inside it?
NMcC "Depending on how edible/toxic you want the stuff to be (i.e. you're adding it to some cells down the line vs just doing spectroscopy), I know of quite a few easily obtainable solvents found at camping or home repair (hardware) or automotive stores."
I want to isolate cellular secretions and harvest intracellular components (which would kill them)
NMcC "Gasoline, diesel, kerosene, ethanol, methanol, limonene (sold as 100% citrus extract air freshener), Methy Ethyl Ketone, Acetone, Naptha, Toluene, Xylene, Mineral Spirits, White Gas (camping), Brake Parts Cleaner (acetone, toluene, methanol, CO2)"
Gasoline, diesel, kerosene, naptha, white gas and mineral spirits aren't pure enough; they're mixtures dozens/hundreds of hydrocarbons, some of which have extremely high boiling points. If you dissolve something in that, you're not getting it back out easily. Limonene also has an extremely high BP. Ethanol, methanol, limonene, methyl ethyl ketone and acetone are water-miscible; I need something that will form a layer with water, not mix with it.
And the aromatics you listed are in that category of reagents that I can't find a manufacturer that will sell to individuals.
Like I said, for purity and low BP, I think dichloromethane is my best bet, assuming those suppliers on Ebay sell to individuals.To view this discussion on the web visit https://groups.google.com/d/msg/diybio/-/ddzhmAfFLQgJ.
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