Re: [DIYbio] What are some good, hydrophobic solvents I can LEGALLY buy?

A 2 liter soda bottle can hold over 100 psi.

At 74 psi, butane is a liquid up to 50 degrees Celsius.

So you could liquefy the butane in a lighter refill, transfer the liquid to a 2 liter bottle with your sample material, and close it up. The pressure won't get above 74 psi, and some of the butane will still be liquid even at room temperature. Put it back in the freezer, and it goes back to liquid at 0 psi, and you can open the bottle.

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On Sat, Jan 5, 2013 at 2:24 PM, Simon Quellen Field <sfield@scitoys.com> wrote:

It's easy to get butane.

It is liquid in your freezer, and boils nicely at room temperature.

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On Sat, Jan 5, 2013 at 2:16 PM, Ulysses1994XF04 <bensics@comcast.net> wrote:

I've been wanting to build a small, home biochemistry lab for a while but the biggest thing holding me back from pouring the time and money into it is that I just can't find any place that will sell the reagents I want to use to individuals (most companies that make laboratory-grade reagents will only sell to certified labs at universities and companies and such).

I really want to do extractions of cellular products (enzymes, pigments, etc) of plants (grass clippings, dead flowers from my garden, etc) and fungi and bacteria I can just grow inside. For some of these, I need a good hydrophobic extraction solvent that's 1) as pure as possible and 2) has a low boiling point so I can easily evaporate/boil off (ideally, I would like to recapture it by distillation so I don't have to constantly buy more of it).

I can't find a single manufacturer that will sell chloroform, dichloromethane or small hydrocarbons like pentane and cyclopentane to individuals. What other hydrophobic solvents can I use?

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