On Sunday, February 24, 2013 3:56:56 AM UTC-8, Mega wrote:
There once was a sonic cleaner in the store for some 20€.I thought about buying it, but won't it damage the plasmids while transforming?????
"Ultrasound treatment for 5–60 s results in plasmid transfer to P. putida UWC1 (P < 0.05). Exposure time of 10 s gives the highest transfer efficiency. No plasmid transfer occurred in the absence of ultrasound treatment. (B) Ultrasound reduced P. putida UWC1 survival following 60 s exposure time, but shorter treatments had little effect on bacterial survival (P < 0.05). (C) Plasmid DNA was prone to cleavage following ultrasound exposure times longer than 30 s"
So yes, you can definitely damage the plasmids and the cells themselves with longer exposure times. But you only need a 10s exposure to get optimal DNA transfer.
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