[DIYbio] Re: This is a shot in the dark, but does anyone happen to love coffee and order lab reagents in bulk?

Here is some advice to get some free stuff :D 

go to www.mobio.com

They have a ton of samples... although I don't know if they will ship to residential address (I have made it a habit of shipping to business address) They have LB agar, broth, plasmid purification kits, gel purification kits and more! All for free! I used that when I was getting into transformations.

 Also I have something that might be helpful that actually came from indiebiotech :) check this out for your transformation-

https://github.com/cathalgarvey/biohacking-protocols/blob/master/E.coli%20Transformation%20With%20PEG%2BMgSO4.md

I would actually recommend this more then the kit because you don't run out of the CaCl mixture (I mean I bought a kg of it but it takes too long to make the concentration right) and all the materials come from your local stores! Then you can go on and use one of their mini prep protocols and purify your plasmid! When I started, I used this plasmid-  pGreen (No not the plant one)

I don't drink coffee often, but I would be willing to give all the help I can :)

Koeng


On Thursday, February 21, 2013 5:39:23 PM UTC-8, Avery Ashley wrote:

So with the help of you guys I'm just getting to the finishing touches of putting my lab together and it's about time to start actually using all this expensive equipment. 

As I'm learning, it can be quite expensive to keep a lab stocked with all the necessary supplies (enzymes, gels and the like), especially when you're quite small and can't order in bulk.

Anyway, I roast coffee as a small business, meaning that I get green coffee beans straight from farms all over the world and "cook" them so they're ready to brew with.

Now I'm just putting this out there for the heck of it, but if anyone would be interested in trading small quantities of lab reagents for freshly roasted coffee I'd be very excited to do so. 

My coffee retails for $12 a pound, but I'd be more than willing to trade a pound of coffee for like $6 (roughly what I buy it at) in supplies so that you come out on the top side. 

I'm not sure if this is viable for anyone on here, or if anyone would even be the slightest bit interested, but why not ask, right?

Of course, I take great pride in my coffee. I get it from the best farms in the world and put extensive time and effort into every pound I roast to ensure it's of the highest quality. I can roast as much or as little as you like, no quantity is too small and no quantity, within shipping constraints, too large. Every pound would be roasted to order the day of shipment as is my policy. 

In closing, I certainly don't want it to seem like I'm asking for a handout. I'm simply offering my services in return for yours.