Re: [DIYbio] Designing primers for PCR

On Fri, Feb 8, 2013 at 2:22 AM, Andreas Sturm <masterstorm123@gmail.com> wrote:
> Ok thanks.
>
>
> I have thought though that the primers were synthesized double stranded, and
> at ~ 60 °C they get denatured, so they also divide into two ssDNA. And then
> do the priming work.

Nope, oligos get synthesized one strand at a time. Primer dimers can
form if the forward and reverse primers have enough complementary
sections.

--
-Nathan

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