Is this going to be used for the glowing plant project?
You could try putting in the Kozak sequence associated with plants, with the start codon, followed by the primer.
NEB has a good program for primer Tm's
AACAATGGC was listed on wiki as terrestrial plant consensus sequence.
This paper also came up on a quick google search but I have no time to read it and the abstract confuses me.
It'll be interesting to see if it works if you ever get it synthesized. I've never designed my own primers, just taken them out of papers or databases, so it's a lacking skill that would be fun to learn.
Are you hoping to use the primers to help get your insert into your pGreen?
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