I like to use SnapGene. Go on their website and get the free trial. It is my all time favorite software for almost anything! It would automatically make the primers from a highlighted sequence, and then you can modify the primer. I highly recommend from primers! Also, a question :). What makes pGreen so special, and so easy to use?
On Friday, February 8, 2013 8:28:15 AM UTC-8, Mega wrote:
Ok thank you!!
I tried it with the primer calculator from ncbi, but I want to include a promoter and RBS in the primer (it's gonna get a quite long primer ;) )
What I saw the online program is not capable of doing this. Is there better software?
So the melting point would not really matter right? It's just that the other primer should have similar melting point?
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