The recipe called for lemon juice and orange was all I had.
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Group: http://groups.google.com/group/diybio/topics
- This is a shot in the dark, but does anyone happen to love coffee and order lab reagents in bulk? [11 Updates]
- DIY restrictive enzymes [1 Update]
- Cubespawn group is working on parallel concepts now [2 Updates]
- Make Your Own Rapid Ligation Kit [2 Updates]
- Is BSA essential in our restriction digests? [3 Updates]
- How can I check a plant is UV resistance or not what are the experiments i can do [1 Update]
- How do you store your DNA constructs? [1 Update]
- clustalw-mpi from ubuntu server 12.04 doesn't work normally [1 Update]
Avery Ashley <avery056056@rams.sccnc.edu> Feb 21 05:39PM -0800
So with the help of you guys I'm just getting to the finishing touches of
putting my lab together and it's about time to start actually using all
this expensive equipment.
As I'm learning, it can be quite expensive to keep a lab stocked with all
the necessary supplies (enzymes, gels and the like), especially when you're
quite small and can't order in bulk.
Anyway, I roast coffee as a small business, meaning that I get green coffee
beans straight from farms all over the world and "cook" them so they're
ready to brew with.
Now I'm just putting this out there for the heck of it, but if anyone would
be interested in trading small quantities of lab reagents for freshly
roasted coffee I'd be very excited to do so.
My coffee retails for $12 a pound, but I'd be more than willing to trade a
pound of coffee for like $6 (roughly what I buy it at) in supplies so that
you come out on the top side.
I'm not sure if this is viable for anyone on here, or if anyone would even
be the slightest bit interested, but why not ask, right?
Of course, I take great pride in my coffee. I get it from the best farms in
the world and put extensive time and effort into every pound I roast to
ensure it's of the highest quality. I can roast as much or as little as you
like, no quantity is too small and no quantity, within shipping
constraints, too large. Every pound would be roasted to order the day of
shipment as is my policy.
In closing, I certainly don't want it to seem like I'm asking for a
handout. I'm simply offering my services in return for yours.
Dakota Hamill <dkotes@gmail.com> Feb 21 09:28PM -0500
Sounds like a neat idea, what are you immediately looking to do? Did you
manage to get everything necessary for PCR and running a gel with gel
visualization? If so I have some primers for fungal and plant ID that
might interest you.
You can find enzymes and agarose and things in pretty small quantities, no
need to buy 10,000 units or 500g really. 200 units for $50 of Taq and $30
for 25g of agarose will each last you ~ 50 PCR rxns and 50 mini gels.
"Daniel C." <dcrookston@gmail.com> Feb 21 10:10PM -0500
On Thu, Feb 21, 2013 at 8:39 PM, Avery Ashley
> Anyway, I roast coffee as a small business, meaning that I get green coffee
> beans straight from farms all over the world and "cook" them so they're
> ready to brew with.
What's more interesting to me is to try to "process" the green coffee
beans with lactobacteria (and maybe a few others? I don't know what
we'd want to use) in an attempt to reproduce in a lab what happens in
the gut of civet cats naturally. You know, those things in southeast
Asia that eat ripe coffee cherries and then poop them out later.
Apparently the bacterial process in their gut changes the flavor
profile quite a bit. If someone could figure out how to do that
artificially I bet you could make a lot of money.
-Dan
Avery Ashley <avery056056@rams.sccnc.edu> Feb 21 07:14PM -0800
As to what I'm immediately looking to do I don't really know. Right now I
just need to learn how to use all these things I've bought haha. My
overarching goal has been to preform a successful bacterial transformation
before the end of summer, so I guess I need to work my way to that.
As far as PCR goes I have everything but microtubes and a reason to do it.
And as of next week I'll be able to run a gel, but I don't have a
visualization system yet.
Really, right now I'd be interested in anything just so I can get up and
running and get my procedures down.
Where do you buy your stuff from? Those prices aren't bad.
On Thursday, February 21, 2013 9:28:04 PM UTC-5, Dakota wrote:
Avery Ashley <avery056056@rams.sccnc.edu> Feb 21 07:16PM -0800
That's a really cool idea. I'm going to look into that.
On Thursday, February 21, 2013 10:10:05 PM UTC-5, Dan wrote:
"Daniel C." <dcrookston@gmail.com> Feb 21 10:21PM -0500
On Thu, Feb 21, 2013 at 10:16 PM, Avery Ashley
> That's a really cool idea. I'm going to look into that.
I think the most difficult part would be identifying the bacterial
profile of the civet cat's gut. I suppose you could just fly there,
shoot one, and empty its intestines into a beaker, but I'm not sure if
that's really the most efficient way to go about it...
-Dan
Avery louie <inactive.e@gmail.com> Feb 21 10:23PM -0500
I would buy some gelgreen from phenix research- they have "sample" sizes
for ~$20. Gelgreen is stable at room temperature, and fluoresces under
blue light. I reccomend getting some cheepo LEDS from amazon- they have a
lot of blue led strips for really cheap. hook them up to like, a 1k
resistor and a 9V and you should be in business.
As for a transformation- that is easy. you can buy a kit from carolina and
do it, or buy a bunch of pieces from all over and do it.
--A
On Thu, Feb 21, 2013 at 10:14 PM, Avery Ashley
Avery Ashley <avery056056@rams.sccnc.edu> Feb 21 07:35PM -0800
I briefly looked into it and it looks like its already been done by the
university of Florida. It's a shame, that would have been cool work.
On Thursday, February 21, 2013 10:21:30 PM UTC-5, Dan wrote:
Avery Ashley <avery056056@rams.sccnc.edu> Feb 21 07:36PM -0800
Cool thanks, I'll take a look at it.
On Thursday, February 21, 2013 10:23:00 PM UTC-5, Avery wrote:
leaking pen <itsatrap@gmail.com> Feb 21 09:26PM -0700
There are already engineered enzyme soaks out there.
Nathan McCorkle <nmz787@gmail.com> Feb 21 09:27PM -0800
I'd be interested, as long as you're a good roaster! I don't have
anything to trade right now, but I'll keep it in mind.
On Thu, Feb 21, 2013 at 5:39 PM, Avery Ashley
--
-Nathan
Koeng <koeng101@gmail.com> Feb 21 09:19PM -0800
Hello all! I was recently looking at restrictive enzymes and wanted to know
if 1 there are any places that offer restrictive enzymes for residential
addresses- and 2 if anyone knows a protocol for DIY restrictive enzyme
purification. I know that edvotek has a kit, but does anyone have any
experience with it? thanks in advance!
Koeng
Bryan Bishop <kanzure@gmail.com> Feb 21 12:55PM -0600
From: John Griessen <john@industromatic.com>
Date: Thu, Feb 21, 2013 at 8:52 AM
Subject: Cubespawn group is working on parallel concepts now
To: biocurious-robo-biohacking@googlegroups.com
Patrick D'H wrote:
"- 180 x 90 cm sounds very large - that's 6 x 3 feet, and would take up an
entire lab bench! Could you get away with 3 x 3 ft, until you have most of
the kinks worked out? Expanding the length of the gantry afterwards should
be reasonably straightforward.
- Were you guys planning to design a separate PCR machine as well? "
There is another project considering what control hardware and software to
use for modular
open manufacturing robots right now that you al should consider cross
pollination with.
It's called Cubespawn. They're discussing using ROS, ARM platforms like
R-Pi, SmoothieBoard
and The basic idea is starting off with modules that are made from T
slotted extrusion
with overall dimensions of 300mm increments.
http://cubespawn.com/wiki/**index.php?title=Main_Page<http://cubespawn.com/wiki/index.php?title=Main_Page>
"System Overview
The CubeSpawn system consists of Cubical frames made from aluminum T-slot
extrusion.
The modular frames are built to enclose various manufacturing machines,
such as a CNC milling machine, or a 3-D printer, or an assembly robot. The
frames are based on a consistent dimensional framework of 300mm increments
and can be linked together, each is an Ethernet appliance, with integrated
power connections.
In this way, up to the limits of the ratings in each branch, cubes can be
joined together to form "production lines""
Dietrich Dehlinger <ddehling@gmail.com> Feb 21 07:17PM -0800
Realistically, we're looking to use some of the same technologies. I don't
think the assembly line philosphy/geometry is right for something like
squidbot (which is way more recursive), but a lot of the same issues are at
play.
On Thursday, February 21, 2013 10:55:18 AM UTC-8, Bryan Bishop wrote:
Mega <masterstorm123@gmail.com> Feb 21 03:24AM -0800
Just found this. It may be helpful to some.
http://www.benchfly.com/video/61/make-your-own-rapid-ligation-kit/
Basically it says, the quick ligase is the same as normal ligase, just the
buffer is different. So changing the buffer will speed it up.
Nathan McCorkle <nmz787@gmail.com> Feb 21 12:11PM -0800
See a previous discussion here:
Ligation, "quick" vs plain/normal
https://groups.google.com/forum/?fromgroups=#!topic/diybio/sXH_LbTYfm8
--
-Nathan
Mega <masterstorm123@gmail.com> Feb 21 01:58AM -0800
Make sure to tell us how it worked ;)
williambeaufoy@gmail.com Feb 21 09:59AM
Will do - has been postponed to saturday (We may have some BSA by then
anyway, but we'll run regardless.)
Andreas Sturm <masterstorm123@gmail.com> Feb 21 12:09PM +0100
Great, thanks ;)
Debadutta Bhoi <debadutta1988@gmail.com> Feb 21 04:11PM +0530
I need anthrocynin quantification procedure by pH differential method. If
any body have please reply or send me links.
--
Debadutta Bhoi
Msc 2nd Yr Life Science
National Institute Of Technology, Rourkela
Odisha
Mega <masterstorm123@gmail.com> Feb 21 02:04AM -0800
What I do to store plasmids at home...
Take a marmelade jar (empty & clean of course). Put in about one centimeter
of water. Freeze it.
Then put the plasmids in.
Because if the freezer does unfreeze (to get the ice away from it's pipes)
cycle, the water acts as a cold sink and keeps the air and the plasmid
cold...
(I don't even know if my freezer can defrost, because it is quite old. But
better on the safe side.
"loïc lauréote" <loic.laureote@gmail.com> Feb 21 10:23AM +0100
ok thanks, this is not something about find an alternative but work for
debugging clustalw-mpi. because i think on a real cluster this kind of lack
of performance could be not visible, if there is a pb, this program have to
be removed from the ubuntu repository.
2013/2/21 ruphos <apokruphos@gmail.com>
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