Re: [DIYbio] Effect of repeated autoclavation

On Tue, Feb 5, 2013 at 12:01 PM, jarlemag <jarle.pahr@gmail.com> wrote:
> Hello. A simple question popped up in the lab today, and I thought perhaps
> some of you would know: Will autoclaving the same medium multiple times
> degrade the quality of common complex growth media such as LB?

I can't speak to the effects on LB, but repeatedly autoclaving growth
medium for Saccharomyces cerevisiae (yeast) can actually improve it by
causing precipitation of chemicals that would otherwise form
particulates and cloud up your final product. Because the important
chemicals are essentially sugar, autoclaving (even multiple times)
won't make the medium less suitable but it can make it progressively
darker in color.

-Dan

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