Re: [DIYbio] Effect of repeated autoclavation

For LB, this isn't so much of an issue, but for media that contain
sugars you can get caramelisation of the sugar, splitting of
disaccharides, or maillard reactions, all of which might interfere with
some sensitive experiments, bacteria, or what have you.

For most experiments though, it won't make much of a difference provided
you try to use the same media batch for everything; doing otherwise, you
might have trouble telling what's different between samples; whether to
blame the bugs or the broth.

On 05/02/13 18:54, Avery louie wrote:
> It can mess up the concentration by removing water, which makes it
> darker as has been mentioned.
>
> --A
>
> On Tue, Feb 5, 2013 at 1:30 PM, Daniel C. <dcrookston@gmail.com
> <mailto:dcrookston@gmail.com>> wrote:
>
> On Tue, Feb 5, 2013 at 12:01 PM, jarlemag <jarle.pahr@gmail.com
> <mailto:jarle.pahr@gmail.com>> wrote:
> > Hello. A simple question popped up in the lab today, and I thought
> perhaps
> > some of you would know: Will autoclaving the same medium multiple
> times
> > degrade the quality of common complex growth media such as LB?
>
> I can't speak to the effects on LB, but repeatedly autoclaving growth
> medium for Saccharomyces cerevisiae (yeast) can actually improve it by
> causing precipitation of chemicals that would otherwise form
> particulates and cloud up your final product. Because the important
> chemicals are essentially sugar, autoclaving (even multiple times)
> won't make the medium less suitable but it can make it progressively
> darker in color.
>
> -Dan
>
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