It would cause a degradation to the agar for sure. Aside from that I've heard some speculation that the proteins in the LB may complex an become unavailable. I know for plant tissue culture MS does not fair well to multi-claves. Not sure exactly what happens but growth is stunted and the evidence points to nutrient deficiency so maybe the ions complex out of solution. Either way without a doubt agar will be messed up.
Sebastian S Cocioba
CEO & Founder
New York Botanics, LLC
Sent via Mobile E-Mail
Hello. A simple question popped up in the lab today, and I thought perhaps some of you would know: Will autoclaving the same medium multiple times degrade the quality of common complex growth media such as LB?
Best regards,
Jarle Pahr
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