Re: [DIYbio] Geneome Sequencing Costs

On Sat, Feb 23, 2013 at 8:13 AM, Mega <masterstorm123@gmail.com> wrote:
> Hi,
>
> Here is an article that says that in 2012 sequencing 1 Mbp costed 0.1$
> already?!?
> http://singularityhub.com/2011/03/05/costs-of-dna-sequencing-falling-fast-look-at-these-graphs/
>
>

How is this done nowadays? I looked at wikipedia on whole-genome
sequencing. They mention nanopore sequencing and pyrosequencing. Are
those methods up and running?

I looked at the shotgun sequencing method. If I understand correctly,
that needs the dna fragments to be subcloned? Won't that take many
hours? You can't just drop some purified DNA and let the machine piece
it together, right?

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