Re: [DIYbio] info on endotoxins

Sydlabs has endo-free silica tubes complete with kit for 10 preps at 160 bucks.

http://www.sydlabs.com/ProductDetails/4-19-152-1356/endofree-plasmid-maxi-kits-spin-column.html

Sebastian S Cocioba
CEO & Founder
New York Botanics, LLC

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On Feb 4, 2013, at 1:14 PM, Jeswin <phillyj101@gmail.com> wrote:

Hey guys,
What can you tell me about endotoxin removal from plasmid maxiprep?
Anyone know of the protocol for using Triton X-114 to remove
endotoxins? It's some sort of aqueous phase separation. I'm not too
sure.

Is there a cheap way to detect endotoxins, e.g., before and after
purification? The gel-clot LAL assay is probably around $200 for 20-30
assays but I just need to test 7 samples.

Thanks

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