Re: [DIYbio] oligo synthesis without an activator?

I don't know much about this besides what's available on wikipedia, but
my understanding is that you keep the activator separate from the next
nucleoside so you can wash away nucleosides before activation of the
next stage.

Assume I barely recall what I'm talking about here:

So, here's the reaction with separate activator / next-N:
~NN + Activator
-> Wash ->
~NN*
-> Wash & add G nucleoside ->
~NNG
-> Wash & add activator ->
~NNG*
-> Wash & add A nucleoside ->
~NNGA

Whereas without the intervening wash steps you could get rapid
polymerisation of new nucleosides:
~NN + Activator + G
-> Wash ->
~NNG
~NNGG
~NNGGGGGGG
-> Add Activator + A nucleoside ->
~NNGAA
~NNGGA
~NNGGGGGGGAAAA

On 02/02/13 16:43, CodeWarrior wrote:
> Alow me to consentrate your atention on the 3rd question. Is there any compelling reason not to premix the activator and nucleoside in an oligo synth cycle. Will there be undesirable side reactions if they are premixed? Also regarding the by products of activated coupling. Are they chemically inert wrt the other chemicals involved or can we expect side reactions if some are present in the premixed activator and nucliocide?
>

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