I'm not totally sure how you mean you prepared the samples, but Triton will absorb strongly in the UV. How did you go about removing the Triton?
On Friday, February 8, 2013 7:04:49 AM UTC-8, phillyj wrote:
On Wed, Feb 6, 2013 at 7:02 PM, Max Berry <maxb...@gmail.com> wrote:--
> I just meant that you may have had to keep the sample at 37C to keep the
> phases separate during centrifugation, but that's assuming they even existed
> in the first place. I'm only guessing, since I've never considered running
Max, I got some weird result in my nanodrop. In a test of DNA with
sodium acetate added, the concentrations after triton extraction are
greater than initial concentration readings. I think something is
interfering with the reading, maybe the salt. Or maybe the salt
changes something so that triton is not fully removed? Any ideas?
Thanks
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