Re: [DIYbio] Re: info on endotoxins

On Wed, Feb 6, 2013 at 6:18 PM, Max Berry <maxberry@gmail.com> wrote:
> Yeah the 15% isopropanol is definitely gonna keep the triton soluble so it
> wouldn't separate - I forgot Qiagen uses isopropanol in those buffers. If
> you do resuspend in water, the phase separation protocol should work in
> that, or you can add a little triton into the load before you put in back on
> the column, which might help on the next run. Actually, it might well have
> separated at 37C even with the isopropanol, but if your centrifuge isn't
> heated then it might have cooled down and resolubilized again.
>

I see. Thanks for the input. I don't understand what you mean about
the heated centrifuge? I centrifuged at room temperature (25-27C) so
you're saying that it resolubilized then? I didn't really notice any
phase after taking it out from the 37C but maybe I wasn't paying
enough attention.

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