Re: [DIYbio] Rolling circle replication-

On Thu, Feb 7, 2013 at 5:48 PM, Koeng <koeng101@gmail.com> wrote:
> Hey I was wondering what exactly makes the DNA "nicked" in the origin. Is it
> just like that? Or is there other enzyme that makes it nicked? This question
> has just been like an itch in my mind
>

Generally when I've heard about plasmids being nicked, it's referring
to damage encountered during an extraction process.

If you're asking how they coil and uncoil during/after replication,
you probably want to start with topoisomerase.

--
-Nathan

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