Re: [DIYbio] Rolling circle replication-

Perhaps, but that would make the plasmid unstable; evolution would
quickly select for a pure culture of plasmid-free bacteria..

On 08/02/13 07:47, Mega wrote:
> But the partner
> monomer resumes its role later in finishing synthesis in such a way
> that
> it permanently inactivates both monomers, preventing catalysis of
> additional plasmids.
>
>
> That sounds like you could engineer the monomer so that it doesn't
> inactivate them, thus getting an infinite plasmid producing machine /
> bacteria. Without any primers.
>
> The bacterium should secrete the plasmids then, else it would burst some
> time :)
>
> --
> -- You received this message because you are subscribed to the Google
> Groups DIYbio group. To post to this group, send email to
> diybio@googlegroups.com. To unsubscribe from this group, send email to
> diybio+unsubscribe@googlegroups.com. For more options, visit this group
> at https://groups.google.com/d/forum/diybio?hl=en
> Learn more at www.diybio.org
> ---
> You received this message because you are subscribed to the Google
> Groups "DIYbio" group.
> To unsubscribe from this group and stop receiving emails from it, send
> an email to diybio+unsubscribe@googlegroups.com.
> To post to this group, send email to diybio@googlegroups.com.
> Visit this group at http://groups.google.com/group/diybio?hl=en.
> To view this discussion on the web visit
> https://groups.google.com/d/msg/diybio/-/OnCr0YgHQEEJ.
> For more options, visit https://groups.google.com/groups/opt_out.
>
>

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio?hl=en.
For more options, visit https://groups.google.com/groups/opt_out.