Re: [DIYbio] This is a shot in the dark, but does anyone happen to love coffee and order lab reagents in bulk?

Cool thanks, I'll take a look at it.

On Thursday, February 21, 2013 10:23:00 PM UTC-5, Avery wrote:

I would buy some gelgreen from phenix research- they have "sample" sizes for ~$20.  Gelgreen is stable at room temperature, and fluoresces under blue light.  I reccomend getting some cheepo LEDS from amazon- they have a lot of blue led strips for really cheap.  hook them up to like, a 1k resistor and a 9V and you should be in business.

As for a transformation- that is easy.  you can buy a kit from carolina and do it, or buy a bunch of pieces from all over and do it.

--A

On Thu, Feb 21, 2013 at 10:14 PM, Avery Ashley <avery...@rams.sccnc.edu> wrote:

As to what I'm immediately looking to do I don't really know. Right now I just need to learn how to use all these things I've bought haha. My overarching goal has been to preform a successful bacterial transformation before the end of summer, so I guess I need to work my way to that. 

As far as PCR goes I have everything but microtubes and a reason to do it. And as of next week I'll be able to run a gel, but I don't have a visualization system yet. 

Really, right now I'd be interested in anything just so I can get up and running and get my procedures down.

Where do you buy your stuff from? Those prices aren't bad.

On Thursday, February 21, 2013 9:28:04 PM UTC-5, Dakota wrote:

Sounds like a neat idea, what are you immediately looking to do?  Did you manage to get everything necessary for PCR and running a gel with gel visualization?  If so I have some primers for fungal and plant ID that might interest you.

You can find enzymes and agarose and things in pretty small quantities, no need to buy 10,000 units or 500g really.  200 units for $50 of Taq and $30 for 25g of agarose will each last you ~ 50 PCR rxns and 50 mini gels.

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