Hi everyone,
I soon need to do a PCR of a ~10 kbp fragment.
I asked in every lab of my university if they had some proofreading polymerase (like Pfu). Because the error rate of Taq polymerase is somewhat like 1:9000, it won't work with > 3 kbp.
Buying one myself may not be an option, because I was told that those are remarkably expensive... (Althought, here it seems quite affordable... http://www.thermoscientificbio.com/pcr-enzymes-master-mixes-and-reagents/pfu-dna-polymerase/ 60€ for 100 units )
Of course, I'd pay the mailing fee, and a little compensation. Or tade it for one of my plasmids. pVIB, pGreenII-0049, p35S-GFP. Have acces to pTracer-CMV2, so I could possibly share also this one.
Just shoot me a mail if interested ;)
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