I don't know if anyone else has posted a review of opentaq yet, so I wanted to put a comment out about it.
I ordered the Taq via express mail for $40/100 rxns +$23 shipping. It was shipped in an overnight envelope at room temperature in a bag with a data/useage sheet. The datasheet was easy to read and well laid out. Due to a mail snafu it did not reach me until 2 days after shipping- when i immediately put it on ice, and took it to the lab.
Unfortunately, I had forgotten my primers, but some people at the pcr hackathon whipped up some test dna with a plate that had been contaminated by fungus, and some draino. The DNA was run against two fungal barcoding primers which we had on hand: ITS1 and TS4, although I don't remember which went where.
In this image: here
from right to left, the lanes are:
- negative control w/ Opentaq
- Barcoding primer w/Opentaq
- Barcoding primer 2 w/Opentaq
- DNA ladder
- negative control
- Barcoding primer w/Neb 2x
- Barcoding primer 2 w/Neb 2x
- Barcoding primer 2 w/Neb 2x
The NEB and Opentaq samples were run on different machines, but this gel shows good amplification using the opentaq master mix, even after it was left out at room temp for a day+
I am impressed and happy with the product. My only criticism is that it would be nice if you got your tracking # with your order.
--Avery
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