Hi everyone,
Bacteria and plastids are capable of having many coding sites per mRNA molecule.
that wold give us a mRNA of 5' cap - RBS - CDS1 - spacer - CDS 2 - spacer - CDS3 - 3'UTR
Now I read the rumor that each CDS needs its own ribosome binding site (SD-sequence)... True?
On the other hand there is this paper saying that for some chloroplast genes, no RBS are needed for expression neither in chloroplasts nor in E.Coli ..
http://www.ncbi.nlm.nih.gov/pubmed/9520261
So what is true when linking two genes? Include another RBS or not??
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