Nice. Looks pretty straightforward:
"By way of example, a particularly preferred solid medium according to this aspect of the invention comprises an absorbent cellulose-based paper such as filter paper having a minimal loading, per sq.cm. of paper, as follows:
(a) EDTA: 0.5 micromols (146.1 mg of free acid)
(b) Tris: 8 micromols (968.8 mg of free base)
(c) SDS: 1 mg; and optionally
(d) uric acid: 2 micromols (336.24 mg of acid)."
(b) Tris: 8 micromols (968.8 mg of free base)
(c) SDS: 1 mg; and optionally
(d) uric acid: 2 micromols (336.24 mg of acid)."
They also mention coating the cards with polystyrene for long-term storage.
Patrik
On Saturday, March 9, 2013 1:41:24 PM UTC-8, Mitch Day wrote:
I have used the Biomatrica DNA storage system for plasmids and bacterial genomic DNA. Yes, it is very over-priced, but it also works well enough. There have been some decent studies that show it is an improvement over desiccated naked DNA or -80C storage.--Another option is FTA paper (Whatman). These are just chemically-treated paper cards. One can apply naked DNA in solution or whole cells to the paper and then recover enough DNA for transformation or PCR. http://www.whatman.com/FTANucleicAcidCollectionStorag eandPurification.aspx There has been a lot more research on FTA paper because it is such a cheap and stable medium. It is still on patent, but not for long.The patent is informative. http://patft.uspto.gov/netacgi/nph-Parser? Sect2=PTO1&Sect2=HITOFF&p=1&u= /netahtml/PTO/search-bool. html&r=1&f=G&l=50&d=PALL& RefSrch=yes&Query=PN/5496562 MY $0.02(USD),Mitch
On Sunday, February 24, 2013 2:21:54 PM UTC-8, Jiri Dluhos wrote:Hello colleagues,please, does anyone have any experience with the commercial DNA capsule services, like DNAcapsule.com or securigene.com?Are their methods of DNA preservation sound? Isn't it just snake oil?I am very tempted to use them for DNA archiving because they don't require constant freezing, but I'm not sure whether the companies really provide what they say. Would I be able to find a difference if they sent me back a vial of salt water in iron case?I will be glad for any opinion.Best regards,Jiri Dluhos
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To post to this group, send email to diybio@googlegroups.com.
Visit this group at http://groups.google.com/group/diybio?hl=en.
To view this discussion on the web visit https://groups.google.com/d/msg/diybio/-/ebmfwACXkSwJ.
For more options, visit https://groups.google.com/groups/opt_out.
0 comments:
Post a Comment