This was one of the applications we looked at for our bioprinter. We decided not to pursue this because IIRC the chemistry is rather toxic.
One important thing to keep in mind that oligo synthesis is really getting commoditized. It's definitely not as cheap as sequencing, but it seems to be growing on a similar exponential trend - just a couple years behind sequencing. So it's highly unlikely you'll be able to throw something together that will be anywhere near competitive with just farming out your oligo synthesis somewhere.
It would definitely be a great learning experience, and you may want to pursue it for that reason alone. But chances are you'll spend orders of magnitude more effort, time and money per oligo than if you just ordered them commercially. And then you'd still have to do sequencing to check that your oligo was synthesized correctly.
Patrik
On Monday, March 11, 2013 7:43:09 AM UTC-7, Koeng wrote:
Hello all! I was recently thinking about how wonderful it would be to be able to synthesize my own primers (Since I do not use restrictive enzymes in cloning). Has anyone done it? I was thinking about the 3D printer to make reactions and perhaps that could work... although I do not exactly know all the steps in synthesis... Anyone please put your 2 cents!--Koeng
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