[DIYbio] Re: Does anyone have a / access to a proofreading polymersase?

Call NEB and ask for a free sample of Q5 https://www.neb.com/products/polymerases-and-amplification-technologies/pcr-polymerases/q5-high-fidelity-dna-polymerases/detail. My lab has gotten about 5 free samples though we can get phusion for $127 per 200 rxn.

On Tuesday, March 19, 2013 2:07:40 PM UTC-4, Mega wrote:

Hi everyone, 

I soon need to do a PCR of a ~10 kbp fragment. 

I asked in every lab of my university if they had some proofreading polymerase (like Pfu). Because the error rate of Taq polymerase is somewhat like 1:9000, it won't work with > 3 kbp. 

Buying one myself may not be an option, because I was told that those are remarkably expensive... (Althought, here it seems quite affordable... http://www.thermoscientificbio.com/pcr-enzymes-master-mixes-and-reagents/pfu-dna-polymerase/ 60€ for 100 units )

Of course, I'd pay the mailing fee, and a little compensation. Or tade it for one of my plasmids. pVIB, pGreenII-0049, p35S-GFP. Have acces to pTracer-CMV2, so I could possibly share also this one. 

Just shoot me a mail if interested ;) 

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