If you are just interested in the acetamidase gene you could consider having it produced synthetically by idt. They will make qblocks - custom 500 bp oligos for $99 if its longer just make it in two sections and ligate, fusion PCR, or gibson them together. I am planning to do this with a codon optimized acetamidase to use in my organism.
On Sunday, March 17, 2013 4:03:08 PM UTC-4, Mega wrote:
Hi,--I'm keen on marker free selection and was just wondering if anyone had this awesome plasmid to share...Could give something in return, such as pGreenII. p35S-GFP, pVIB from a brand new midiprep that I got a lot of glowing colonies with, ... On filte paper or in an eppi. Or some other compensation ;)
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