[DIYbio] Re: Improving E.coli dehydration tolerance

A great thought... 


 by regularly drying out the culture and culturing the survivors

That actually could work! 

Well I'm kind of doing that. Not regularily, But I froze E.Coli+pVIB and rethawed them now (nearly a year or so later).

There was 1 glowing colonies and lots of other colonies which didn't glow... Contamination? From the 10% glycerol I added probably. But as long as one colony carries the plasmid everything is fine!  

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